4-Hidroksi-3-metilbut-2-enil difosfat reduktaza
| 4-hidroksi-3-metilbut-2-enil difosfat reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.17.1.2 | ||||||||
| CAS broj | 512789-14-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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4-hidroksi-3-metilbut-2-enil difosfat reduktaza (EC 1.17.1.2, izopentenil-difosfat:NADP+ oksidoreduktaza, LytB, (E)-4-hidroksi-3-metilbut-2-en-1-il difosfatna reduktaza, HMBPP reduktaza, IspH, LytB/IspH) je enzim sa sistematskim imenom izopentenil-difosfat:NAD(P)+ oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) izopentenil difosfat + NAD(P)+ + H2O (E)-4-hidroksi-3-metilbut-2-en-1-il difosfat + NAD(P)H + H+
- (2) dimetilalil difosfat + NAD(P)+ + H2O (E)-4-hidroksi-3-metilbut-2-en-1-il difosfat + NAD(P)H + H+
Ovaj enzim je gvožđe-sumporni protein koji sadrži bilo [3Fe-4S] ili [4Fe-4S] kluster. Enzim sačinjava sistem u kome se feredoksin prvo redukuje i naknadno se reoksiduje posredstvom NAD(P)+-zavisne reduktaze.
- ↑ Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A. and Eisenreich, W. (2002). „Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein”. Proc. Natl. Acad. Sci. USA 99: 1158-1163. PMID 11818558.
- ↑ Hintz, M., Reichenberg, A., Altincicek, B., Bahr, U., Gschwind, R.M., Kollas, A.-K., Beck, E., Wiesner, J., Eberl, M. and Jomaa, H. (2001). „Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli”. FEBS Lett. 509: 317-322. PMID 11741609.
- ↑ Charon, L., Pale-Grosdemange, C. and Rohmer, M. (1999). „On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis”. Tetrahedron Lett. 40: 7231-7234.
- ↑ Röhrich, R.C., Englert, N., Troschke, K., Reichenberg, A., Hintz, M., Seeber, F., Balconi, E., Aliverti, A., Zanetti, G., Köhler, U., Pfeiffer, M., Beck, E., Jomaa, H. and Wiesner, J. (2005). „Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum”. FEBS Lett. 579: 6433-6438. PMID 16289098.
- ↑ Wolff, M., Seemann, M., Bui, T.S.B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A. and Rohmer, M. (2003). „Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein”. FEBS Lett. 541: 115-120. PMID 12706830.
- ↑ Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., Schramek, N., Schleicher, E., Weber, S., Haslbeck, M., Buchner, J., Rieder, C., Arigoni, D., Bacher, A., Eisenreich, W. and Rohdich, F. (2004). „IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis”. J. Am. Chem. Soc. 126: 12847-12855. PMID 15469281.
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